Molecular Physiology, Biophysics and Pharmacology of

 SUR/KIR6 and Disorders of Metabolism-Excitation Coupling


Here are some images of the Babenko Lab at the Pacific NW Research Institute. Check out our "arsenal".

This electrophysiological setup was used to characterize pancreatic beta-cells from SUR1-null mice


This patch-clamp setup is used for single-channel recording and analysis of stochastic transitions that gate ion channels. It includes a fluorescence module to visualize tagged subunits, and a rapid solution changer to determine ligand doze-responses. All the devices are controlled via PCs communicating with the server. Many parts of this setup were custom made. We thank Russian Foundation for Fundamental Investigations, INSERM, American Heart Association, and NIH for their support, making possible to obtain numerous pieces of equipment for this setup.

 

The entrance to "The Dark Space", where we build our new advanced microscopy system



that will include Nikon Perfect Focus System and laser TIRF/fast smFRET modules
.


This is our shared Leica DM6000B/CTR7000-based Ca2+ microfluorometry setup.


We also share this Olympus IX81-based fluorescence laser scanning microscopy system.


This is the Babenko's new wet laboratory space, including molecular biology & biochemistry benches,


equipped with ABI's fast PCR, Bio-Rad electroforesis systems, etc. We also acquired



this fast Real Time PCR System from ABI, StepOnePlus. We also share 


these ABI Prism 310 Genetic Analyzers, this Packard COBRA II Auto Gamma Counter,


this Beckman LS 6500 Multi-purpose Scintillation Counter,


this Perkin Elmer Wallac Victor2 1420 Multilabel Counter,


this HORIBA Fluorolog spectrophotometer,


this complete Warner Planar Lipid Bilayer Workstation,


and this Bio-Rad Gene Pulser II Electroporation System.


This fully equipped cell tissue culture room is adjacent to the wet lab space and


the common area with this ELGA Purelab/Barnstead NANOpure Infinity water purification combo,


several
ultra low temperature freezers and refrigerators, as well as


these Sorvall RC M120CX/Evolution and Beckman J2-HS/J-6M/TL-100?Optima XL-80K ultracentrifuges.


We have free access to other general laboratory equipment and instruments around, including

this Savant Speed Vac Plus SC110A/Novalyphe-NL150 vacuum pump system,


these Bio-Rad 583 Gel Dryers,


this FOTODYNE Foto/Analyst 'Investigator' with 60-2030 Coomassie Blue and 60-2031 Ethidium Bromide Filters,


this EG&G Berthold Lumam LB9507,


this New Brunswick BioFlo/CelliGen 115,


and this Pharmacia FPLC System. 


Moreover, adjacent to the Babenko Lab space on the fourth floor, there are


this commonly used Ice-O-Matic machine from Welbilt Company and


this cold room, well equipped for a protein biochemistry work.


 In this warmer room, our research scientists can analyze and discuss data which they collected.

 

Finally, here the PI is reviewing, modeling, designing, writing, submitting... screaming, rebuttling, loosing hope, and, after all, finding solutions -- to celebrate this life just a few times a year by opening his narrow office window...


...and enjoying that wide perspective!


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